Ariadne: Database Search for RNA Identification Using Tandem Mass Spectrometry Data

Ariadne is a web-based database search service for the identification of RNAs and their post-transcriptional modifications using tandem mass spectrometry data.If you include results from Ariadne in a publication, please cite the Ariadne paper.


Parts Editor

Mass Calculator

MS/MS Search

Browse demo search results of some of our published data including ribosomal RNA, transfer RNA, microRNA and other non-coding RNAs and more. Download and try example MS/MS data to search RNA sequence databases by yourself.

Searching an RNA sequence database (up to 50MB) with post-transcriptional modifications using up to 50MB of MS/MS data in Mascot generic format (MGF).

To Guest Users: Enter the Search ID issued at the search to browse the result report.


Calculating mass values of a nucleic acid, its RNase digests and their CID fragments from given sequence(s).

Defining/editing user nucleotide parts such as Nucleosides, Linkers, and Termini as well as unusual part-specific MS/MS dissociations.

Table of the available parts that can be used in a calculation/search.