MS/MS Search

Peak List File (in Mascot Generic Format: MGF)

Product Intensity Cutoff

Absolute: Relative:

Sequences and Modifications

Input Directly into Text Box

Load FASTA and Modification Files

FASTA File:

Modification File (Optional, .mods):

Intact Termini:

The Terminus parts can be specified in FASTA sequences. The priority is symbols in FASTA sequence > those in modification file (.mod) > those selected in the form.

5′ Term: 3′ Term:

Mapping Type:

Max Number of Variable Terminal Truncations 5′ : 3′ :

Enzyme: Max Missed Cleavages:

Parameters

Polarity:

Ion Activation Type:

Mass Tolerance: ± ppm

MS/MS Tolerance: ± ppm

Filter Type: Significance Level:

Reject Length for Mapping:

MS/MS Search

Peak List File (in Mascot Generic Format: MGF)

Sequences and Modifications

Parameters

Isotope Set