MGF File: 160427tRNAphe50f04_spice11 FASTA File: Sc_tRNA_site_direct_mod.fasta Modification File: - Partial Modification: off Intact 5′ Term: p Intact 3′ Term: OH Enzyme: RNaseT1 Max Missed Cleavages: 2 Polarity: negative Ion Activation Type: HCD/CID Mass Tolerance: 10 MS2 Tolerance: 20 Filter Type: Rank first and Above threshold Significance Level: 0.01 Reject Length for Mapping: 2 Isotope Set: natural