Product Intensity Cutoff Relative 0.00001 Product Intensity Cutoff Absolute 10 Polarity Negative Isotope Set Natural 5′ Term OH 3′ Term OH Enzyme No Enzyme Max Missed Cleavages 0 Max Number of Variable Terminal Truncations 5′ 0 Max Number of Variable Terminal Truncations 3′ 0 Mass Tolerance 500 MS/MS Tolerance 20 Ion Activation Type HCD/CID Significance Level 0.01 Reject Length for Mapping 2 Filter Type Rank First and above Threshold Mapping Type Fixed Database synthetic_siRNA.fasta MGF File synthetic_siRNA_Waters_Q-Tof_MaxEnt3.mgf User ID koike Search ID e34cd8766a230e11fb3d90f0a96ee5af