MGF File: 160427tRNAphe50f04_spice11 FASTA File: Sc_tRNA_site_direct_mod.fasta Modification File: - Partial Modification: Off 5′ Term: p 3′ Term: OH Max Number of Variable Terminal Truncations 5′: 0 Max Number of Variable Terminal Truncations 3′: 0 Enzyme: RNase T1 Max Missed Cleavages: 2 Polarity: Negative Ion Activation Type: HCD/CID Mass Tolerance: 10 MS/MS Tolerance: 20 Filter Type: Rank First and above Threshold Significance Level: 0.01 Reject Length for Mapping: 2 Isotope Set: Natural