Ariadne: Database search for RNA identification using tandem mass spectrometry data

Ariadne is a web-based database search service for the identification of RNAs and their post-transcriptional modifications using tandem mass spectrometry data.If you include results from Ariadne in a publication, please cite the Ariadne paper.


Browse demo search results of some of our published data including ribosomal RNA, transfer RNA, microRNA and other non-coding RNAs and more. Download and try example MS/MS data to search RNA sequence databases by yourself.

Searching an RNA sequence database (up to 50MB) with post-transcriptional modifications using up to 50MB of MS/MS data in Mascot generic format (MGF).

Browse search result

A result report can be browsed with 'search ID' issued on the time of search.


calculating mass values of a nucleic acid, its RNase digests and their CID fragments from given sequence(s).

Available nucleosides, linkers, and termini in calculation / search are listed.

The registered user can browse search results afterward as a list.Contact the Ariadne Development Team via e-mail to get your user ID.